Raman spectroscopy and atomic force microscopy as tools to evaluate the radiobiological sensitivity of breast cancer cells at different therapeutic doses

Radiotherapy is widely used in the treatment of breast cancer (BC), particularly in aggressive subtypes such as triple negative breast cancer, where therapeutic options are limited. Gamma (γ) radiation induces the production of reactive oxygen species (ROS) through water radiolysis, which can alter cells by triggering biochemical and biomechanical changes [1]. The radiation effects at cellular level such as molecular and membrane alterations can provide predictive biomarkers, which may be useful for devising more individualized strategic treatment.