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A 25-year-old man is accused of stabbing a stranger with a broken bottle in the bathroom of a Duluth nightclub because he thought the victim is gay. Kyle Eugene Heisler Jr., of Deer River, has been charged with second-degree assault with a dangerous weapon, threats of violence, and obstruction. The alleged incident unfolded shortly after midnight Sunday at Aces on First in downtown Duluth. According to a criminal complaint, Heisler confronted the victim, a 37-year-old man, at the urinal in the bar’s bathroom and directed “homophobic slurs” at him and his friend, then stabbed him with a broken Busch Light bottle. St. Louis County The victim sustained 2- to 3-inch slash wound on the left side of his abdomen and several puncture wounds on his chest. When he was taken to a hospital, doctors determined that the man’s kidney had been punctured. Heisler was identified as the attacker and arrested somewhere near Aces on First minutes after officers were dispatched. Officers noted that he appeared to have blood on his shirt. The complaint says Heisler was “highly combative” during his arrest and “made numerous threats” to officers, including that he would put the arresting officer “in the dirt” and that he would “get” the investigating officer upon his release. The victim had gone to the bar with his wife and friends to dance after they attended a wedding reception. None of them were aware of any interaction with Heisler before the stabbing, indicating that it was motivated by his “brief observation” of the man dancing with the others. As reported by the Duluth News Tribune, a prosecutor believes the attack was “based solely on a cursory and incorrect assessment of the person’s sexual orientation.” St. Louis County prosecutor Mike Ryan described Heisler as “a threat to virtually anyone who crosses his path,” and said his “conduct evinces a willingness to resort to extreme violence with very little motivation.” Since it may have been a bias-motivated crime, it’s possible that additional charges will be filed against Heisler, whose bail was set at $100,000.

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by Wei Feng, Boya Li, Yiqiong Niu, Qiannan Xu, Xiandun Zhai The zebrafish (Danio rerio) is a widely used vertebrate model in biomedical research, yet the rapid and cost-effective extraction of genomic DNA (gDNA) from adult zebrafish tissue remains a practical challenge for routine molecular applications. To address this, we systematically optimized and evaluated a Chelex-100/proteinase K-based extraction protocol across eight adult zebrafish tissues (muscle, liver, heart, spleen, kidney, intestine, brain, and eggs) and compared its performance against that of a commercial kit. The optimized protocol, involving incubation at 56 °C for 15 min and boiling at 100 °C for 10 min, required approximately 25 min per sample. Across all tested tissues (3 biological replicates per tissue), the Chelex-100 method yielded gDNA concentrations ranging from 177.2 ± 3.5 ng/µL to 674.5 ± 22.1 ng/µL per 5 mg of tissue, which were generally higher than those obtained with the commercial kit. However, the purity (A260/A280 ratios) was consistently lower for the Chelex-100 method (1.36–1.83) than for the kit (1.84–2.02), indicating the coextraction of proteinaceous impurities. Despite this, Chelex-extracted gDNA supported successful PCR amplification and Sanger sequencing of target fragments, with consistent amplicon quality. In real-time PCR assays, both methods produced amplification curves, but the Chelex-100 method yielded systematically higher Ct values, suggesting reduced sensitivity with less-pure templates. Crucially, the Chelex-100 method avoided hazardous organic solvents and required no column purification, with an estimated reagent cost of <$0.20 per sample versus approximately $2.00 per sample for the commercial kit. We conclude that this optimized Chelex-100/proteinase K protocol provides a rapid, economical, and operationally simple alternative for routine gDNA extraction from adult zebrafish tissues and is particularly suitable for PCR-based genotyping, sequencing and qPCR applications, though its lower purity warrants careful consideration for high-sensitivity quantitative assays.

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by Weijian Liu, Jianbo Li, Zhizeng Zhang, Zhenxia Yuan, Shuai Teng, Danqing Song, Mingrui Zhu, Wenlong Lv To address the issues of reduced microseismic signal detection accuracy and difficulty in identifying event boundaries under low signal-to-noise ratio conditions, we propose SEU-Net, an automatic microseismic signal segmentation model that integrates a Squeeze-and-Excitation (SE) attention mechanism. This model embeds an SE module within the U-Net encoder, which enhances feature responses related to microseismic event boundaries through adaptive re-scaling of channel features. Simultaneously, it employs a temporal signal dimension expansion method to convert one-dimensional waveforms into an input format suitable for two-dimensional convolutional networks, thereby enabling end-to-end detection of microseismic event start and end times. Validation results using rock uniaxial compression acoustic emission test data show that SEU-Net achieves an overall segmentation accuracy of 98.97% on the test set, representing an improvement of approximately 1 percentage point over U-Net. Under a signal-to-noise ratio (SNR) of 5 dB, the proportions of samples with start and end point detection errors falling within the 0–5 range were 79.98% and 89.93%, respectively. Compared with EQTP, SEU-Net achieved an initial F1 score of 88.88% and an intersection-to-union ratio of 79.98%, which are higher than EQTP’s 86.81% and 76.69%, respectively. The error distribution and various evaluation metrics indicate that SEU-Net can maintain high accuracy in event boundary detection even under noise interference. Three-dimensional source localization based on the detection results shows that the spatial distribution of sources corresponding to SEU-Net is more concentrated and consistent with the actual fracture evolution process of the test specimen. This method provides technical support for the automatic detection of microseismic signals and source localization in complex rock mass engineering environments with low signal-to-noise ratios.

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An aide at a Cherry Hill nursing home pleaded guilty to spraying bleach in the face of a paralyzed patient, New Jersey Attorney General Jennifer Davenport said Thursday.